1.  Enhancement of circulation

There is decreased oxygen absorption in circulatory disease due to reduced surface area, This is a result of hindered blood flow through the small capillaries after clumping of red blood cells. Ozone is beneficial for restoring red cell flexibility, reducing or eliminating clumping and enabling oxygen carrying ability. There is oxygenation of the tissues as viscosity decreases and arterial partial pressure increases. Blood vessels are unclogged and plaque in the arteries is oxidized with Ozone

2. Stimulation of oxygen metabolism

There is an increased rate of red blood cell glycolysis caused by Ozone. 2,3-diphosphoglycerate (2,3-DPG) is stimulated which increases the release of the amount of oxygen to the tissues. The production of ATP is stimulated once there is activation of the Krebs cycle through enhancing oxidative carboxylation of pyruvate after Ozone. The NADH reducing process increases after Ozone which helps to oxidize cytochrome C. There are a number of enzymes produced after being stimulated for production by Ozone, These enzymes act as free radical scavengers and cell wall protectors. Ozone induces production of prostacyclin which is a vasodilator and a platelet aggregation inhibitor.

3. Formation of peroxides

Hydro peroxides are formed when Ozone reacts with unsaturated fatty acids of the lipid layer in cellular membranes. A synergistic effect is created with cellular-formed H2O2. Alkanes, alkenes, carbonyls, ozonides, carbonides, singlet oxygen, alkoxyl and peroxyl radicals are products of lipid peroxidation.

4. Activation of the immune system

The production of interferon, tumor necrosis factor (TNF) and interleukin 2 is at its greatest when Ozone is administered at a concentration of between 30 and 55 ug/cc. Interleukin 2 productions has an entire cascade of subsequent immunological reactions.